Sungchul Hohng 

Sungchul Hohng

Department of Physics and Astronomy

Department of Biophysics and Chemical Biology

Seoul National University

Tel: 82-2-880-6593

Fax: 82-2-884-3002

E-mail: shohng@snu.ac.kr

EDUCATION

2000: Ph.D. in Physics, Seoul National University, Korea.

Thesis: Studies of anomalous anti-Stokes and Stokes transfer phenomena in seimconductor heterostructures (mentor: Dai-Sik Kim)

1996: M.S. in Physics, Seoul National University, Korea.

1994: B.S. in Physics, Seoul National University, Korea.

WORK EXPERIENCE

2015 – present: Professor, Department of Physics and Astronomy, Seoul National University

2010 – 2015: Associate Professor, Department of Physics and Astronomy, Seoul National University

2006 – 2010: Assistant Professor, Department of Physics and Astronomy, Seoul National University.

2002 – 2006: Postdoctoral Research Associate (mentor: Taekjip Ha), Department of Physics, Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign.

2001: Postdoctoral Research Associate (mentor: Dai-Sik Kim), Department of Physics, Seoul National University, Korea.

2000: Postdoctoral Research Associate (mentor: Wonho Jhe) Center for Near-field Atom-photon Technology, Seoul National University, Korea.

RESEARCH INTERESTS

Genome Maintenance; Transcription & Chromatin Remodeling; RNA Biology; Development of Novel Single-Molecule and Superreolution Imaging Techniques.

PUBLICATIONS

81. Soochul Shin1, Kwangbeom Hyun, Jinwoo Lee, Dongwon Joo, Tomasz Kulikowicz, Vilhelm A. Bohr, Jaehoon Kim and Sungchul Hohng, “Werner syndrome protein works as a dimer for unwinding and replication fork regression”, Nucleic Acids Research (accepted).

80. Juyoung Kim, Chanshin Kang, Soochul Shin & Sungchul Hohng, "Rapid quantification of miRNAs using dynamic FRET-FISH", Communications Biology (2022).

79. Eunho Song, Heesoo Uhm, Palinda Ruvan Munasingha, Seungha Hwang, Yeon-Soo Seo, Jin Young Kang, Changwon Kang & Sungchul Hohng, "Rho-dependent transcription termination proceeds via three routes", Nature Communications (2022).

78. Gunhyoung Lim and Sungchul Hohng, "Single-molecule Fluore.scence Technique to Monitor the Co-transcriptional Formation of G-quadruplex and R-loop Structures", Bio-protocol (2021).

77. Eunhye Lee, Chanshin Kang, Pasi Purhonen, Hans Hebert, Karim Bouazoune, Sungchul Hohng, and Ji-Joon Song "A Novel N-terminal Region to Chromodomain in CHD7 is Required for the Efficient Remodeling Activity ", Journal of Molecular Biology (2021).

76. Eitan Lerner, et. al. "FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices ", Elife (2021).

75. Wooyoung Kang, Seungha Hwang, Jin Young Kang, Changwon Kang, and Sungchul Hohng "Hopping and Flipping of RNA Polymerase on DNA during Recycling for Reinitiation after Intrinsic Termination in Bacterial Transcription", International Journal of Molecular Sciences (2021).

74. Jaewon Kirk, Ju Yeon Lee, Yejin Lee, Chanshin Kang, Soochul Shin, Eunhye Lee, Ji-Joon Song, and Sungchul Hohng "Yeast Chd1p Unwraps the Exit Side DNA upon ATP Binding to Facilitate the Nucleosome Translocation Occurring upon ATP Hydrolysis", Biochemistry (2020).

73. Soochul Shin, Yoonseok Jung, Heeso Uhm, Minseok Song, Soomin Son, Jiyoung Goo, Cherlhyun Jeong, Ji-Joon Song, V. Narry Kim and Sungchul Hohng "Quantification of purified endogenous miRNAs with high sensitivity and specificity", Nature Communications (2020).

72. Gunhyoung Lim, Sungchul Hohng "Single-molecule fluorescence studies on cotranscriptional G-quadruplex formation coupled with R-loop formation", Nucleic Acids Research (2020).

71. Wooyoung Kang*, Kook Sun Ha*, Heesoo Uhm, Kyuhyong Park, Ja Yil Lee, Sungchul Hohng*, Changwon Kang*, “Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA” Nat Communications (2020).

70. Heesoo Uhm, Sungchul Hohng, “Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding” Methods Mol Biol (2020).

69. Park SH, Kang N, Song E, Wie M, Lee EA, Hwang S, Lee D, Ra JS, Park IB, Park J, Kang S, Park JH, Hohng S, Lee KY, Myung K. “ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress” Nat Communications (2019).

68. Jang S, Kang C, Yang HS, Jung T, Hebert H, Chung KY, Kim SJ, Hohng S, Song JJ. “Structural basis of recognition and destabilization of the histone H2B ubiquitinated nucleosome by the DOT1L histone H3 Lys79 methyltransferase” Genes Dev (2019).

67. Jongjin Lee, Sangjun Park, Sungchul Hohng, “Accelerated FRET-PAINT microscopy”, Mol Brain (2018).

66. Soochul Shin*, Kwangbeom Hyun*, Jaehoon Kim, and Sungchul Hohng “ATP Binding to Rad5 Initiates Replication Fork Reversal by Inducing the Unwinding of the Leading Arm and the Formation of the Holliday Junction”, Cell Reports (2018).

65. Mina Lee*, Soochul Shin*, Heesoo Uhm*, Heesun Hong, Jaewon Kirk, Kwangbeom Hyun, Tomasz Kulikowicz, Jaehoon Kim, Byungchan Ahn, Vilhelm A Bohr and Sungchul Hohng "Multiple RPAs make WRN syndrome protein a superhelicase", Nucleic Acid Research (2018).

64. Sangjun Park*, Wooyoung Kang*,Yeong-Dae Kwon, Jaehoon Shim, Siyong Kim, Bong-Kiun Kaang and Sungchul Hohng "Superresolution fluorescence microscopy for 3D reconstruction of thick samples", Mol Brain (2018).

63. Seung-Ryoung Jung, Eunji Kim, Soochul Shin, Ji-Joon Song, and Sungchul Hohng “Mechanisms of the binding/dissociation acceleration of the target-guide interaction by Thermus thermophilus Argonaute”, Bull. Korean Chem. Soc. 31, 1021 (2018).

62. Jongjin Lee, Sangjun Park, Wooyoung Kang, and Sungchul Hohng, “Accelerated super-resolution imaging with FRET-PAINT”, Mol Brain (2017).

61. Heesoo Uhm, Wooyoung Kang, Kook Sun Ha, Changwon Kang, Sungchul Hohng, “Single-molecule FRET studies on the cotranscriptional folding of a thiamine pyrophosphate riboswitch”, Proc. Natl. Acad. Sci. U.S.A. (2017).

60. Minhyeok Chang, Jungsic Oh, Yeonghoon Kim, Sungchul Hohng and Jong-Bong Lee, “Extended depth of field for single biomolecule optical imaging-force spectroscopy”, Opt Express (2017).

59. Nam-Kyung Yu, Heesoo Uhm, Jaehoon Shim, Jun-Hyeok Choi, Sangsu Bae, Todd Charlton Sacktor, Sungchul Hohng*, Bong-Kiun Kaang*, “Increased PKMζ activity impedes lateral movement of GluA2-containing AMPA receptors”, Mol Brain (2017).

58. Heesoo Uhm and Sungchul Hohng, “Ligand Recognition Mechanism of Thiamine Pyrophosphate Riboswitch Aptamer”, Bull. Korean Chem. Soc. 31, 1021 (2017).

57. Hyun Huh, Jinwoo Lee, Hyung Jun Kim, Sungchul Hohng, and Seong Keun Kim, “Morphological analysis of oligomeric vs. fibrillar forms of a-synuclein aggregates with super-resolution BALM imaging”, Chem. Phys. Lett. (2017).

56. Ju Yeon Lee, Robert J. Lake, Jaewon Kirk, Vilhelm A. Bohr, Hua-Ying Fan, and Sungchul Hohng, "NAP1L1 accelerates activation and decreases pausing to enhance nucleosome remodeling by CSB", Nucleic Acids Research (2017).

55. Kyung-Won Min*, Myung Hyun Jo*, Soochul Shin*, Sylvia Davila, Richard W. Zealy,Soo Im Kang, Lawson T. Lloyd, Sungchul Hohng, and Je-Hyun Yoon, "AUF1 facilitates microRNA-mediated gene silencing", Nucleic Acid Research (2017).

54. Myung Hyun Jo, Sungchul Hohng, "Single-Molecule Fluorescence Energy Transfer Assays for the Characterization of Reaction Pathways of miRNA-Argonaute Complex", Methods Mol Biol (2017).

53. Soochul Shin, Jinwoo Lee, Sangwoon Yoo, Tomasz Kulikowicz, Vilhelm A. Bohr, Byungchan Ahn, Sungchul Hohng, "Active Control of Repetitive Structural Transitions between Replication Forks and Holliday Junctions by Werner Syndrome Helicase", Structure (2016).

52. S. Lee, Y. Jang, S.-J.Lee, S.Hohng, "Single-Molecule Multicolor FRET Assay for Studying Structural Dynamics of Biomolecules", Methods in Enzymology (2016).

51. Heesoo Uhm, Sangsu Bae, Mina Lee and Sungchul Hohng, "Single-Molecule FRET Combined with Magnetic Tweezers at Low Force Regime", Bulletin of the Korean Chemical Society (2016).

50. S. Chul Kwon, Tuan Anh Nguyen, Yeon-Gil Choi, Myung Hyun Jo, Sungchul Hohng, V. Narry Kim and Jae-Sung Woo, "Structure of Human DROSHA", Cell (2016).

49. Myung Hyun Jo, Ji-Joon Song, and Sungchul Hohng, "Single-molecule fluorescence measurements reveal the reaction mechanisms of the core-RISC, composed of human Argonaute 2 and a guide RNA", BMB Rep. (2015).

48. Je-Hyun Yoon, Myung Hyun Jo, Elizabeth J.F. White, Supriyo De, Markus Hafner, Beth E. Zucconi, Kotb Abdelmohsen, Jennifer L. Martindale, Xiaoling Yang, William H. Wood III, Yu Mi Shin, Ji-Joon Song, Thomas Tuschl, Kevin G. Becker, Gerald M. Wilson, Sungchul Hohng, and Myriam Gorospe, “AUF1 promotes let-7b loading on Argonaute 2”, Genes & Development (2015).

47. Myung Hyun Jo*, Soochul Shin*, Seung-Ryoung Jung, Eunji Kim, Ji-Joon Song, and Sungchul Hohng, "Human Argonaute 2 has diverse reaction pathways on target RNAs", Molecular Cell (2015).

46. Tuan Anh Nguyen, Myung Hyun Jo, Yeon-Gil Choi, Joha Park, S. Chul Kwon, Sungchul Hohng, V. Narry Kim, Jae-Sung Woo, "Functional Anatomy of the Human Microprocessor", Cell (2015).

45. Soonsil Hyun, Areum Han, Myung Hyun Jo, Sungchul Hohng, and Jaehoon Yu, "Dicer Nuclease-Promoted Production of Let7a-1 MicroRNA Is Enhanced in the Presence of Tryptophan-Containing Amphiphilic Peptides", ChemBioChem (2014).

44. Moon-Hyeong Seo, Jeongbin Park, Eunkyung Kim, Sungchul Hohng & Hak-Sung Kim, "Protein conformational dynamics dictate the binding affinity for a ligand", Nature Communications (2014).  

43. Doyoun Kim, Jeonghwan Hur, Kwangsoo Park, Sangsu Bae, Donghyuk Shin, Sung Chul Ha, Hye-Yeon Hwang, Sungchul Hohng, Joon-Hwa Lee, Sangho Lee, Yang-Gyun Kim, and Kyeong Kyu Kim, "Distinct Z-DNA binding mode of a PKR-like protein kinase containing a Z-DNA binding domain (PKZ)", Nucleic Acid Research (2014).

42. Sanghwa Lee and Sungchul Hohng, "An Optical Trap Combined with Three-Color FRET", J. Am. Chem. Soc (2013).

41. Seung-Ryoung Jung, Eunji Kim, Wonseok Hwang, Soochul Shin, Ji-Joon Song, and Sungchul Hohng, "Dynamic Anchoring of the 3′-End of the Guide Strand Controls the Target Dissociation of Argonaute–Guide Complex", J. Am. Chem. Soc (2013).

40. Sangsu Bae, Yuyoung Kim, Doyoun Kim, Kyeong Kyu Kim, Yang-Gyun Kim, and Sungchul Hohng, "Energetics of Z-DNA Binding Protein-Mediated Helicity Reversals in DNA, RNA, and DNA–RNA Duplexes", J. Phys. Chem. B (2013).

39. Juhun Park, Jinwoo Lee, Seon Namgung, Kwang Heo, Hyungwoo Lee, Sungchul Hohng, and Seunghun Hong, "Sub-Diffraction Limit Imaging of Inorganic Nanowire Networks Interfacing Cells", Small (2013).

38. Sangsu Bae, Heyjin Son, Yang-Gyun Kim and Sungchul Hohng, "Z-DNA stabilization is dominated by the Hofmeister effect", Physical Chemistry Chemical Physics (2013).

37. Hohng S, Lee S, Lee J, Jo MH. "Maximizing information content of single-molecule FRET experiments: multi-color FRET and FRET combined with force or torque", Chemical Society Reviews (2013).

36. Ta HM, Bae S, Han S, Song J, Ahn TK, Hohng S, Lee S, Kim KK. "Structure-based elucidation of the regulatory mechanism for aminopeptidase activity", Acta Crystallographica Section D (2013).

35. Kim E, Lee S, Jeon A, Choi JM, Lee H-S, Hohng S, Kim H-S. "A single-molecule dissection of ligand binding to a protein with intrinsic dynamics", Nature Chem Biol (2013).

34. Hwang W, Bae S, Hohng S. “Autofocusing system based on optical astigmatism analysis of single-molecule images”, Opt Express 20, 29353 (2012).

33. Peisley A, Jo MH, Lin C, Wu B, Orme-Johnson M, Walz T, Hohng S, Hur S. “Kinetic mechanism for viral dsRNA length discrimination by MDA5 filaments”, Proc. Natl. Acad. Sci. U.S.A. (2012).

32. Lee J, Miyanaga Y, Ueda M, Hohng S. “Video-rate confocal microscopy for single-molecule imaging in live cells and super-resolution fluorescence imaging.”, Biophys. J. 103, 1691 (2012).

31. Hyeon C, Lee J, Yoon J, Hohng S, Thirumalai D. “Hidden complexity in the isomerization dynamics of Holliday junctions”, Nat. Chem. 4, 907 (2012).

30. Lee S, Jung S-R, Heo K, Byl JAW, Deweese JE, Osheroff N, Hohng S. "DNA cleavage and opening reactions of human topoisomerases IIα are regulated via Mg2+-mediated dynamic bending of gate-DNA", Proc. Natl. Acad. Sci. U.S.A. 109, 2925 (2012).

29. Yeom K-H, Heo I, Lee J, Hohng S, Kim VN, Joo C. “Single-molecule approach to immunoprecipitated protein complexes: insights into miRNA uridylation”, EMBO Report 12, 690 (2011).

28. Hwang W, Arlusion V, Hohng S. “Dynamic competition of DsrA and rpoS fragments for the proximal binding site of Hfq as a means for efficient annealing”, Nucl. Acids. Res. 39, 5131 (2011).

27. Bae S, Kim D, Kim KK, Kim Y-G, Hohng S. “Intrinsic Z-DNA is stabilized by the conformational selection mechanism of Z-DNA-binding proteins”, J. Am. Chem. Soc. 133, 668 (2011).

26. Lee J, Lee S, Ragunathan K, Joo C, Ha T, Hohng S. “Single-molecule four-color FRET”, Angew. Chem. Int. Ed. 49, 9922 (2010).

25. Lee S, Lee J, Hohng S. “Single-molecule three-color FRET with both negligible spectral overlap and long observation time”, PLoS ONE 5,e12270 (2010).

24. Yoon J, Kang HJ, Sung J, Park HJ, Hohng S. “Highly Polymorphic G-quadruplexes in the c-MYC promoter”, Bull. Korean Chem. Soc. 31, 1025 (2010).

23. Hong H, Kim Y-G, Hohng S. “Single-molecule FRET studies on frameshifting RNA structures of human immunodeficiency virus”, Bull. Korean Chem. Soc. 31, 1021 (2010).

22. Roy R, Hohng S, Ha T. “A practical guide to single-molecule FRET”, Nat. Meth. 5, 507 (2008).

21. Hohng S, Zhou R, Nahas MK, Yu J, Schulten K, Lilley DMJ, Ha T. “Fluorescence-force spectroscopy maps two-dimensional reaction landscape of the Holliday junction”, Science 318, 279 (2007).

20. Hohng S, Arlusion V, Roy R, Pellegrini O, Regnier P, Ha T. “Spectroscopic observation of RNA chaperone activities of Hfq in post-transcriptional regulation by a small non-coding RNA”, Nucl. Acids Res. 35, 999 (2007).

19. Hohng S, Ha T. “Single-molecule quantum dot FRET”, ChemPhysChem 6, 956 (2005).

18. Nahas MK, Wilson TJ, Hohng S, Lilley DMJ, Ha T. “Observation of internal cleavage and ligation reactions of a ribozyme”, Nat. Struct. Mol. Biol. 11, 1107 (2004).

17. Hohng S, Joo C, Ha T. “Single-molecule three-color FRET”, Biophys. J. 87, 1328 (2004).

16. Brunner R, Burkhardt M, Pesch A, Sandfuchs O, Ferstl M, Hohng S, White JO. “Diffraction based solid immersion lens” J. Opt. Soc. Am. A 21, 1186 (2004).

15. Hohng S, Ha T. “Near-complete suppression of quantum dot blinking in ambient conditions”, J. Am. Chem. Soc. 126, 1324 (2004).

14. Hohng S, Wilson T, Tan E, Clegg RM, Lilley DMJ, Ha T.  “Conformational flexibility of four-way junctions in RNA”, J. Mol. Biol. 336, 69 (2004).

13. Kim DS, Hohng SC, Malyarchuk V, Yoon YC, Ahn YH, Yee KJ, Park JW, Kim J, Park QH, Lienau C. “Microscopic origin of surface-plasmon radiation in plasmonic band-gap nanostructures”, Phys. Rev. Lett. 91, 143901 (2003).

12. Hohng SC, Yoon YC, Kim DS, Malyarchuck V, Müller R, Lienau C, Park JW, Yoo KH, Kim J, Ryu HY, Park QH. “Light emission from the shadows: Surface plasmon nano-optics at near and far fields”, Appl. Phys. Lett. 81, 3239 (2002).

11. Jho YD, Lee JY, Yahng JS, Yee KJ, Hohng SC, Kim DS. “Real-to-virtual transition in bulk GaAs: the thickness dependence”, J. Kor. Phys. Soc. 37, 728 (2000).

10. Yee DS, Yee KJ, Hohng SC, Kim DS, Meier T, Koch SW. “Coherent control of absorption and polarization decay in a GaAs quantum well: Time and spectral domain studies”, Phys. Rev. Lett. 84, 3474 (2000).

9. Hohng SC, Khang DW, Ahn YH, Lee JY, Kihm SY, Kim DH, Kim WS, Woo JC, Kim DS, Citrin DS, Woo DH, Kim EK, Kim SH, Lim KS. “Two color picosecond and CW experiments on the anti-Stokes and Stokes carrier transfer phenomena in GaAs/AlxGa1-xAs and InGaP2/AlxGa1-xAs heterostructures”, Phys. Rev. B 60, 8883 (1999).

8. Hohng SC, Kim DS. “Two-color picosecond experiments on anti-Stokes photoluminescence in GaAs/AlGaAs asymmetric double quantum wells”, Appl. Phys. Lett. 75, 3620 (1999).

7. Ahn YH, Yahng JS, Sohn JY, Yee KJ, Hohng SC, Woo JC, Kim DS, Meier T, Koch SW, Lim YS, Kim EK. “From exciton resonance to frequency mixing in the GaAs multiple quantum wells”, Phys. Rev. Lett. 82, 3879 (1999).

6. Kim DS, Sohn JY, Yahng JS, Ahn YH, Yee KJ, Yee DS, Jho YD, Hohng SC, Kim DH, Kim WS, Woo JC, Meier T, Koch SW, Woo DH, Kim EK, Kim SH, Kim CS. “Femtosecond four-wave mixing experiments on GaAs quantum wells using two independently tunable lasers”, Phys. Rev. Lett. 80, 4803 (1998).

5. Kim DS, Ko HS, Kim YM, Rhee SJ, Hohng SC, Yee YH, Kim WS, Woo JC, Choi HJ, Ihm J, Woo DH, Kang KN. “Anomalous real space charge transfer through thick barriers in GaAs/AlxGa1-xAs  asymmetric double quantum wells: AlxGa1-xAs as a percolating barriers”, Solid State Commun. 100, 231 (1996).

4. Kim DS, Ko HS, Kim YM, Rhee SJ, Hohng SC, Yee YH, Yee DS, Khim JS, Kim WS, Woo JC. “Anti-Stokes luminescence in a coupled system: a natural consequence of quantum oscillation”, Prog. Cryst. Growth Ch. 33, 161 (1996).

3. Kim DS, Ko HS, Kim YM, Rhee SJ, Hohng SC, Yee YH, Yee DS, Woo JC, Choi HJ, Ihm J, Woo DH, Kang KN. “Thick AlxGa1-xAs: An intrinsically percolating barrier owing to its microscopic structural inhomogeneity”, Appl. Phys. Lett. 69, 2513 (1996).

2. Kim DS, Ko HS, Kim YM, Rhee SJ, Hohng SC, Yee YH, Kim WS, Woo JC, Choi HJ, Ihm J, Woo DH, Kang KN. “Percolation of carriers through low potential channels in thick AlxGa1-xAs (x < 0.35) barriers”, Phys. Rev. B 54, 14580 (1996).

1. Kim DS, Ko HS, Lim YS, Kim YM, Lee JS, Rhee SJ, Kim WS, Hong SC, Yee YH, Khim JS, Jung JM, Huhr S, Lee JH, Chang JS, Choe BD, Woo JC, Song PH, Choi HJ, Jhi SH, Ihm J, Shin EJ, Kim D, Woo DH, Kang KN, Song JJ. “Going beyond the mean-field approximations of alloys and alloy superlattices: a few puzzles solved”, J. Opt. Soc. Am. B 13, 1210 (1996).